Accurate, precise, rapid and economical UV Spectrophotometric first derivative method was developed for the estimation of Ampicillin Trihydrate and Cloxacillin Sodium in suspension dosage form. The principle for Area under Curve is “the area is measured around wavelength maxima of drug”. Area selected for Quantitation was 214.0-236.0 nm for Ampicillin (AMP) and 215.0-240.0 nm for Cloxacillin (CLO) in 0.1 N NaOH. Linearity observed in the concentration range was 10-30 µg/ml and 10-30 µg/ml for AMP and CLO respectively. The accuracy and precision were determined and found to comply with ICH guidelines.
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